Collection and freezing of equine epididymal spermatozoa

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Collection and freezing of equine epididymal spermatozoa

The first report of a foal born after artificial insemination (AI) with frozen/thawed semen, more than fifty years ago, was the result of insemination with epididymal spermatozoa (Barker en Gandier, 1957). Ever since, reports have confirmed pregnancies and/ or births after AI with epididymal semen, which proves the fertilizing capacity of epididymal semen (Morris, 2002; Monteiro et al., 2011). ...

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Immunogenicity of Testicular and Epididymal Spermatozoa

Problem: The sequential changes in the immunogenicity of spermatozoa in male reproductive tract and the effect of seminal vesicle secretions are long thought to act as central players in influencing immunological equilibrium in the male reproductive tract. Method of Study: Popliteal lymph nodes of mice were collected on the 8th day after sensitizing them with the testicular and epididymal sperm...

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Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell begins to elongate and differentiate into what is morphologically recognized as a spermatozoon. However, the spermatozoon is very immature, having no ability for motility or egg recognition. Both of these events occur o...

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Leptin Receptor in Ram Epididymal Spermatozoa

This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then...

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Immunological differentiation of epididymal and seminal spermatozoa of the rabbit.

Rabbit spermatozoa from the epididymis lack the antigenic material present on seminal spermatozoa, which these latter cells have in common with the seminal plasma. This observation provides further support for the indirect evidence, obtained previously, that antigenic material is taken up by the spermatozoa from the seminal plasma.

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ژورنال

عنوان ژورنال: Vlaams Diergeneeskundig Tijdschrift

سال: 2014

ISSN: 0303-9021,0303-9021

DOI: 10.21825/vdt.v83i6.16631